A ubiquitin-independent proteasome pathway controls activation of the CARD8 inflammasome

CARD8 is a pattern-recognition receptor that forms a caspase-1-activating inflammasome. CARD8 undergoes constitutive autoproteolysis, generating an N-terminal (NT) fragment with a disordered region and a ZU5 domain and a C-terminal (CT) fragment with UPA and CARD domains. Dipeptidyl peptidase 8 and dipeptidyl peptidase 9 inhibitors, including Val-boroPro, accelerate the degradation of the NT fragment via a poorly characterized proteasome-mediated pathway, thereby releasing the inflammatory CT fragment from autoinhibition. Here, we show that the core 20S proteasome, which degrades disordered and misfolded proteins independent of ubiquitin modification, controls activation of the CARD8 inflammasome. In unstressed cells, we discovered that the 20S proteasome degrades just the NT disordered region, leaving behind the folded ZU5, UPA, and CARD domains to act as an inhibitor of inflammasome assembly. However, in Val-boroPro–stressed cells, we show the 20S proteasome degrades the entire NT fragment, perhaps due to ZU5 domain unfolding, freeing the CT fragment from autoinhibition. Taken together, these results show that the susceptibility of the CARD8 NT domain to 20S proteasome-mediated degradation controls inflammasome activation.

CARD8 is a pattern-recognition receptor that forms a caspase-1-activating inflammasome. CARD8 undergoes constitutive autoproteolysis, generating an N-terminal (NT) fragment with a disordered region and a ZU5 domain and a C-terminal (CT) fragment with UPA and CARD domains. Dipeptidyl peptidase 8 and dipeptidyl peptidase 9 inhibitors, including Val-boroPro, accelerate the degradation of the NT fragment via a poorly characterized proteasome-mediated pathway, thereby releasing the inflammatory CT fragment from autoinhibition. Here, we show that the core 20S proteasome, which degrades disordered and misfolded proteins independent of ubiquitin modification, controls activation of the CARD8 inflammasome. In unstressed cells, we discovered that the 20S proteasome degrades just the NT disordered region, leaving behind the folded ZU5, UPA, and CARD domains to act as an inhibitor of inflammasome assembly. However, in Val-bor-oPro-stressed cells, we show the 20S proteasome degrades the entire NT fragment, perhaps due to ZU5 domain unfolding, freeing the CT fragment from autoinhibition. Taken together, these results show that the susceptibility of the CARD8 NT domain to 20S proteasome-mediated degradation controls inflammasome activation.
Several intracellular danger-associated signals induce the assembly of multiprotein complexes called inflammasomes (1,2). The typical process of inflammasome formation involves a pattern recognition receptor (PRR) protein detecting a specific danger signal, self-oligomerizing, and then recruiting (directly or indirectly via the adapter protein ASC) the cysteine protease caspase-1 (CASP1). CASP1 undergoes proximityinduced autoproteolysis on this platform, generating an active enzyme that cleaves and activates gasdermin D (GSDMD) and, in most cases, interleukin-1β (IL-1β) and IL-18. The N-terminal fragment of cleaved GSDMD (GSDMD p30 ) forms pores in the cell membrane, releasing the activated cytokines and triggering pyroptotic cell death.
CARD8 is a human PRR that forms an inflammasome (3). CARD8 has an N-terminal unstructured region consisting of 160 amino acids followed by a function-to-find domain and a caspase activation and recruitment domain (CARD) (Fig. 1A). The function-to-find domain undergoes autoproteolysis between its ZU5 (found in ZO-1 and UNC5) and UPA (conserved in UNC5, PIDD, and ankyrins) subdomains, creating N-terminal (NT) and C-terminal (CT) fragments that remain noncovalently associated (4). The proteasomemediated degradation of the NT fragment releases the CT fragment from autoinhibition, but the CT fragment is then captured and restrained as part of a ternary complex with one copy of full-length CARD8 and one copy of dipeptidyl peptidase 8 or 9 (DPP8/9) (5). Stimuli that accelerate CARD8 NT degradation and/or disrupt the DPP8/9-CARD8 complex enable the CARD8 CT to overcome these repressive mechanisms and to self-oligomerize, recruit CASP1, and trigger pyroptosis.
Two distinct danger signals have been reported to accelerate the proteasome-mediated degradation of the CARD8 NT fragment. First, HIV-1 protease directly cleaves within the NT region of the CARD8 FL protein, generating an unstable neo-Nterminus that is rapidly degraded by the N-end rule proteasome pathway (6). Second, DPP8/9 inhibitors, including Val-boroPro (VbP), accelerate the degradation of many disordered and misfolded proteins, including the CARD8 NT (7,8). Notably, DPP8/9 inhibitors also destabilize the repressive DPP8/9-CARD8 ternary complex (5) and thereby activate the CARD8 inflammasome via two separate mechanisms.
The molecular details of the homeostatic and DPP8/9inhibition induced CARD8 degradation pathways have not been established. Intracellular proteins are often degraded by the ubiquitin-proteasome system, which involves the covalent attachment of ubiquitin to lysine residues on target proteins that mediate their recruitment to the 26S proteasome. The 26S proteasome consists of the proteolytic core 20S subunit capped at one or both ends by 19S regulatory complexes (9). The 19S regulatory particles recognize, deubiquitinate, and unfold target proteins, enabling their translocation into the 20S core particle for hydrolysis. In a preliminary attempt to identify sites of ubiquitination on CARD8, we previously mutated all 10 lysines within the NT fragment of CARD8 FL to arginines    Figure 1. CARD8's disordered region is removed in cells. A, domain organization of CARD8. CARD8 undergoes autoproteolysis between the ZU5 and UPA subdomains. The size in kilodaltons (kDa) of each fragment is indicated. B, lysates of the indicated cell lines were analyzed by immunoblotting. FL, CARD8 full-length; CT, CARD8 C-terminal fragment; p44, CARD8 44 kDa species; asterisks (*) denote nonspecific bands. C, HEK 293T cells were transfected with plasmids encoding the indicated FLAG-tagged constructs (left). Lysates were analyzed by immunoblotting (right). D, the specific residues of CARD8 that the antibodies target are depicted on the cartoon (top). HEK 293T cells were transiently transfected with a plasmid encoding C-terminally HA-tagged CARD8 FL WT construct and lysates were analyzed by immunoblotting (bottom). E, C-terminally FLAG-tagged CARD8 FL WT expressed from HEK 293T cells were purified with anti-FLAG beads. The 44 kDa ponceau-stained band was analyzed by Edman degradation (right). pm, picomoles. F, Edman degradation analysis in E (CARD8 FL K10R). We found that CARD8 FL K10R was largely, but not completely, insensitive to VbP in a reconstituted HEK 293T cell system expressing CASP1 and GSDMD (HEK 293T CASP1+GSDMD cells), suggesting that ubiquitination of the NT fragment might be important for VbP-induced degradation (8). However, the CARD8 FL K10R protein expressed at lower levels and underwent less autoproteolysis than the CARD8 FL WT protein, and these deficiencies could also account for its reduced pyroptotic activity.
Here, we further investigated the molecular details of CARD8 degradation. We found that the core 20S proteasome, which degrades misfolded proteins independent of the 19S regulatory complex and ubiquitination, regulates CARD8 activation. In unstressed cells, the 20S proteasome removes the disordered region of CARD8, leaving behind the folded ZU5-UPA-CARD (ZUC) domains. This protein fragment cannot form an inflammasome but can still sequester CT fragments in the DPP8/9 ternary complex and thereby act as an inflammasome inhibitor. In VbP-stressed cells, the 20S proteasome degrades CARD8's entire NT fragment, including the ZU5 domain, releasing the inflammatory CT fragment from autoinhibition. Collectively, these findings suggest that the propensity of the ZU5 domain to enter the 20S proteasome is a critical regulatory step that governs the activation of the CARD8 inflammasome.

CARD8's disordered region is removed in cells
Before further studying VbP-induced CARD8 NT degradation, we first wanted to investigate how CARD8 is processed in unstressed cells. Intriguingly, we and others have consistently observed that endogenous CARD8 in human monocytes appears as three distinct bands at 61, 44, and 27 kDa in immunoblots using antibodies targeting CARD8 CT (Fig. 1B) (3,4,10). Only a fraction of the CARD8 FL typically undergoes autoproteolysis (4,11,12), and the bands at 61 kDa and 27 kDa correspond to CARD8 FL and CARD8 CT , respectively. The identity of the third band at 44 kDa, which we call CARD8 p44 , however, is unknown. CARD8 p44 is unlikely to be a splicing isoform, as the transfection of the cDNA encoding the canonical 61 kDa CARD8 isoform (i.e., isoform 5) in HEK 293T cells also generated CARD8 p44 (Fig. 1C, lane 3). Notably, ectopic expression of the autoproteolysis-defective S297A mutant CARD8 FL protein (CARD8 FL S297A) similarly generated CARD8 p44 (Fig. 1C, lane 5), demonstrating that formation of this species does not depend on autoproteolysis. We predicted that CARD8 p44 corresponded to CARD8 FL protein that was N-terminally truncated prior to its ZU5 domain because it migrated slightly more slowly than the isolated ZU5-UPA-CARD domains (CARD8 ZUC ) (Fig. 1C, lanes 4 and 6) and was not detected with antibodies targeting CARD8 NT (Fig. 1D).
Indeed, Edman degradation analysis revealed that the N-terminal residue of CARD8 p44 was F150 (Fig. 1, E and F). Thus, a fraction of CARD8 FL is proteolytically cleaved 12 residues before the start of the ZU5 domain to generate CARD8 p44 . However, it should be noted that in some instances, we observed CARD8 fragments slightly larger than CARD8 p44 , suggesting that processing does not exclusively occur at this single site (Fig. 1, B-D). In addition, CARD8 ZUC also appears to further be processed into a p26 fragment, but the identity and function of this band was not studied further here (Fig. 1C, lane 4).
We previously evaluated the abilities of several N-terminally truncated CARD8 proteins to mediate VbP-induced pyroptosis in HEK 293T CASP1+GSDMD cells (8). In this analysis, we discovered a CARD8 construct starting at K147, but not F150, was capable of mediating pyroptosis. Notably, the construct that starts at F150 (i.e., CARD8 Δ1-149 ) essentially mimics CARD8 p44 , and therefore this analysis suggested that CARD8 p44 cannot form an inflammasome. We next wanted to confirm this result in THP-1 cells, which endogenously express the CARD8 inflammasome pathway and are therefore more physiologically relevant (3). To do this, we ectopically expressed CARD8 FL WT, CARD8 FL S297A, or CARD8 Δ1-149 in CARD8 -/-THP-1 cells before treating the cells with DMSO or VbP (Fig. 1G). As expected, VbP induced pyroptosis in THP-1 cells expressing CARD8 FL WT, but not CARD8 FL S297A or CARD8 Δ1-149 . The aminopeptidase inhibitor bestatin methyl ester (MeBs) synergizes with VbP to induce more pyroptosis (7), but the combination of VbP and MeBs still failed to activate CARD8 Δ1-149 (Fig. 1H). Collectively, these data show that CARD8 p44 cannot form an inflammasome.
The CARD8 ZUC can occupy the CARD8 FL position in the CARD8-DPP8/9 ternary complex and thus can capture and repress a freed CARD8 CT fragment (5). As such, we predicted that CARD8 p44 , which is only slightly longer than CARD8 ZUC , might function as an inhibitor of inflammasome formation. To test this idea, we expressed CARD8 FL with an N-terminal degradation tag (dTAG-CARD8 FL ) in HEK 293T CASP1+GSDMD cells ( Fig. 1I) (5,13). The small molecule dTAG-13 triggers the rapid degradation of proteins fused to dTAGs, and therefore treatment of these cells with dTAG-13 induced the release of free CARD8 CT and pyroptosis. Consistent with our hypothesis, the coexpression of CARD8 Δ1-149 in these cells abolished pyroptosis without impacting dTAG-13-induced dTAG-CARD8 FL degradation. Overall, these results suggest that an endogenous protease removes CARD8's disordered region and generates an inhibitory form of CARD8 that blocks inflammasome activation.

The proteasome generates CARD8 p44
We next wanted to determine the sequence and structural requirements for the proteolysis of CARD8 FL into CARD8 p44 .
revealed residues F150 to Y156 (colored red) as the N-terminus of CARD8 p44 . F150 is shown on the CARD8 structure predicted by Alpha-Fold (26). G-H, CARD8 -/-THP-1 cells stably expressing CARD8 WT, S297A, or Δ1-149 were treated with compounds for 5 h (G) or 3 h (H) before LDH release and immunoblotting analyses. ****p < 0.0001, by Student's two-sided t test. NS, not significant. I, HEK 293T CASP1+GSDMD cells were transfected with plasmids encoding the indicated proteins. After 24 h, cells were treated with dTAG-13 for 5 h before LDH release and immunoblot analyses. Data in (G-I) (n = 3) are means ± standard deviation (SD) of replicates. All data, including immunoblots, are representative of three or more independent experiments. HA, hemagglutinin. LDH, lactate dehydrogenase.
We previously created the chimeric protein MTMR1 M1-Q94 -CARD8 ZUC , in which the disordered region of CARD8 was replaced by the disordered region (residues M1 to Q94) of MTMR1 (8). Intriguingly, the expression of this chimeric protein in HEK 293T cells still generated a p44 fragment ( Fig. 2A, lane 3). The disordered regions of MTMR1 and CARD8 do not share any homology (Fig. 2B), indicating that the unknown protease does not recognize and cleave a specific   Ubiquitin-independent CARD8 regulation amino acid sequence. To determine if the CARD8 ZUC specifically directs the cleavage, we appended the disordered region of CARD8 on the N terminus of GFP. Interestingly, we found that that the disordered region was similarly removed to generate a protein species with a molecular weight close to GFP's ( Fig. 2C). In addition, we found that appending a wellfolded GFP tag to the N-terminus of CARD8 FL or CARD8 Δ1-130 did not interfere with CARD8 p44 generation ( Fig. 2A, lanes  4 and 5), showing that the unknown protease has endopeptidase activity (i.e., it can remove disordered regions between well-folded domains). In contrast, replacement of the entire CARD8 disordered region with GFP (GFP-CARD8 ZUC ) abolished CARD8 p44 generation ( Fig. 2A, lane 6). Notably, an antibody targeting GFP detected bands corresponding to GFP itself for the GFP-CARD8 chimeras in which some disorder is present (i.e., GFP-CARD8 FL and GFP-CARD8 Δ1-130 ) but not from the GFP-CARD8 ZUC protein (Fig. 2D, lanes 2 to 4). It should be noted that the protease typically removed the disordered region entirely from these chimeric proteins to generate fragments corresponding to the ordered domains but that larger fragments with partial disordered region removal were often observed as well (Fig. 2, A, C, and D). Overall, these data indicate that the CARD8-cleaving protease removes disordered regions in a sequence independent manner, including disordered regions between two well-folded domains. Intriguingly, the proteolytic 20S core of the proteasome, which often functions independently of the 19S cap in cells, degrades unstructured polypeptides regardless of amino acid sequence, including those between structured domains (14)(15)(16). As such, we hypothesized that the 20S proteasome removed the disordered regions from CARD8 and the chimeric proteins. As this process is likely processive, it would also account for the higher molecular weight fragments that we observed in our immunoblotting analyses (i.e., the larger fragments likely correspond to proteins that were not completely digested). To test this idea, we transiently transfected HEK 293T cells with a doxycycline (DOX)-inducible construct encoding autoproteolysis-defective CARD8 FL S297A, treated cells with DOX and with increasing concentrations of the proteasome inhibitor bortezomib, and assayed for newly formed CARD8 p44 by immunoblotting (Fig. 2E). We observed that bortezomib blocked the generation of CARD8 p44 , strongly indicating that the proteasome was indeed responsible for this cleavage.
The 26S proteasome typically requires the covalent attachment of ubiquitin to substrate proteins preceding their degradation, whereas the 20S proteasome directly degrades misfolded proteins without ubiquitination (14)(15)(16). To determine whether CARD8 p44 formation involves lysine ubiquitination, we mutated all lysines to arginines within the NT fragment (CARD8 FL K10R) or throughout the entire protein (CARD8 FL K26R). It should be noted that the CARD8 FL K26R protein is entirely devoid of lysines, including its C-terminal linker and hemagglutinin (HA)-tag. We observed that both mutant proteins still generated CARD8 p44 fragments in HEK 293T cells and that bortezomib still attenuated the formation of these products (Fig. 2, F and G). These results suggest that the 20S proteasome removes the disordered region of CARD8 through a ubiquitin-independent mechanism.
The 20S proteasome generates CARD8 p44 in vitro As proteasome inhibition might indirectly block CARD8 p44 formation in cells, we next sought to confirm that the purified 20S proteasome directly removes CARD8's disordered region to generate CARD8 p44 in vitro. We therefore purified a C-terminally FLAG-tagged CARD8 FL protein from HEK 293T cells using anti-FLAG beads and then incubated this protein with purified 20S proteasomes. We found that the 20S proteasome robustly degraded CARD8 FL into CARD8 p44 but that the presumably well-folded CARD8 p44 and CARD8 CT products were largely protected from degradation (Fig. 3A). It should be emphasized that the purified 20S proteasome generated a p44 band precisely the same size as the one present from constitutive processing by endogenous proteasomes, strongly indicating that the 20S proteasome generates CARD8 p44 in cells. In addition, the 20S proteasome also generated slightly larger CARD8 species in this experiment, consistent with the idea that it processively degrades CARD8 in an N to C direction. As expected, the proteasome inhibitors bortezomib and MG-132 slowed the in vitro generation of CARD8 p44 (Fig. 3B). Like CARD8 p44 and CARD8 CT , we found that the CARD8 ZUC was resistant to 20S proteasome-mediated degradation for at least 4 h (Fig. 3C).
As mentioned above, the 20S proteasome has previously been shown to degrade disordered sequences but spare wellfolded domains (14,15). To determine if the 20S proteasome could indeed process the chimeric proteins evaluated above, we purified several of these proteins from HEK 293T cells and similarly incubated them with purified 20S proteasomes. As expected, we found that the purified 20S proteasome efficiently removed the CARD8 disordered region (M1-F161) fused to the N terminus of GFP (Fig. 3D), as well as the MTMR1 disordered region (M1-Q94) fused to the N terminus of CARD8 ZUC (Fig. 3E, lane 6). Also as expected, the 20S proteasome exhibited endoproteolytic activity, generating CARD8 p44 even when a GFP-tag was appended to the N terminus of CARD8 FL (Fig. 3E, lane 8). In stark contrast, GFP-CARD8 ZUC , a chimeric protein that lacks the internal disordered region, was resistant to 20S proteasome-mediated degradation (Fig. 3E, lane 10). Lastly, 20S proteasomes processed the CARD8 FL K10R and K26R proteins into p44 fragments, in agreement with the known capability of the 20S to degrade substrates without lysines or ubiquitination (Fig. 3F).

VbP activates CARD8 lacking NT lysines
Interestingly, the 20S proteasome did not always appear to generate substantially more of CARD8 p44 from CARD8 FL in vitro (e.g., Fig. 3E, lanes 1 and 2; Fig. 3F, lanes 1 and 2), suggesting that degradation may proceed through the ZU5 domain in some cases. Moreover, VbP does not induce visible ubiquitination of CARD8 by immunoblotting (Fig. 4A) (3,5,7,8,10). We therefore wanted to determine if the 20S proteasome also mediates VbP-induced degradation of the entire CARD8 NT fragment, the key process that releases the pyroptotic CARD8 CT fragment from autoinhibition. To further explore this idea, we more closely investigated the ability of CARD8 FL K10R to stimulate pyroptosis. As mentioned above, we previously found that CARD8 FL K10R was largely defective in mediating VbP-induced pyroptosis in the reconstituted HEK 293T CASP1+GSDMD system (8), but this inactivity was perhaps due to compromised autoproteolysis or expression. Here, we instead stably expressed CARD8 FL K10R in the more physiologically relevant CARD8 -/-THP-1 cells. We observed that VbP, and especially the combination of VbP and MeBs, induced pyroptosis, as evidenced by lactate dehydrogenase (LDH) release and GSDMD cleavage, in cells expressing CARD8 FL K10R (Fig. 4B). Moreover, we found that bortezomib (and caspase-1 inhibitor VX-765) abolished CARD8 FL K10R-dependent pyroptosis, showing that the lysine-free CARD8 NT fragment was indeed being degraded by the proteasome. As expected, neither the autoproteolysis-defective CARD8 FL K10R/S297A nor the p44 fragment of CARD8 FL K10R (CARD8 Δ1-149 K3R) formed inflammasomes.
In theory, it remained possible that VbP induces the ubiquitination of the CT fragment of the autoproteolyzed CARD8 FL K10R protein and that this ubiquitination is sufficient to recruit CARD8 to the 26S proteasome and trigger its degradation in an N to C direction. To test this idea, we next    Figure 3. The 20S proteasome generates CARD8 p44 in vitro. A, purified CARD8 FL WT protein were incubated at varying dilutions with or without purified 20S proteasomes for 4 h. Reactions were quenched with 2× loading dye prior to immunoblotting analysis. B, purified 20S proteasomes were preincubated with the indicated vehicle or proteasome inhibitors for 1 h, prior to adding purified CARD8 FL WT for an additional 1 h. Reactions were quenched with 2× loading dye prior to immunoblotting analysis. C, purified CARD8 FL and CARD8 ZUC (each 800 nM) were incubated with purified 20S proteasomes (100 nM). At the indicated timepoints, aliquots were removed from the mixture, quenched with 2× loading dye, and analyzed by immunoblotting. D-F, the indicated purified proteins were treated and analyzed as described in (A). Immunoblots are representative of three or more independent experiments. CARD8-HA CARD8 THP-1 (8h)  investigated the ability of the lysine-free CARD8 FL K26R protein to mediate pyroptosis. We found that neither VbP nor the combination of VbP and MeBs induced pyroptosis in CARD8 -/-THP-1 cells ectopically expressing CARD8 FL K26R (Fig. 4, B and C). However, this protein was likely inactive for at least two reasons. First, CARD8 FL K26R was severely defective in autoproteolytic activity (Fig. 4D, lanes 2 and 3;  Fig. S1A lanes 3 and 6; densitometry ratio of CT:FL in CARD8 FL WT and K26R are 60% and 6%, respectively). Second, the isolated CT fragment of this construct (which contains 16 lysines mutated to arginines) was impaired in its ability to oligomerize into a functional inflammasome, as transient transfection of considerably more plasmid encoding this mutant CT fragment relative to the WT CT fragment was required to induce GSDMD cleavage in HEK 293T CASP1+GSDMD cells (Fig. 4E). Moreover, despite these deficiencies, CARD8 FL K26R still retained binding to DPP9 and acted as an inflammasome inhibitor in the ternary complex (Fig. S1, B and C). Collectively, these results suggest that the inability of CARD8 FL K26R to mediate pyroptosis is not necessarily due to a lack of ubiquitination sites. Because this lysine-free protein is nonfunctional for several reasons, it is unfortunately not possible to unequivocally demonstrate that VbP-induced NT degradation is independent of lysine ubiquitination. We previously discovered that the NEDD8-activating enzyme inhibitor MLN4924 (17) blocks VbP-induced CARD8 activation (8). Cullin E3 ligases require neddylation for their activity, and we therefore speculated that a cullin E3 ligase might ubiquitinate CARD8. Notably, the activity of MLN4924 is the only evidence that suggests that CARD8's degradation requires ubiquitination. Here, we found that MLN4924 blocks VbP-induced pyroptosis in CARD8 -/-THP-1 cells expressing CARD8 FL K10R (Fig. S1D). As this protein has no lysines on its NT, we reasoned that MLN4924 treatment does not inhibit CARD8 inflammasome activation by directly abrogating CARD8 ubiquitination. Rather, we reasoned that its inhibitory activity may be due to an indirect disruption of 20S proteasome activity. For example, MLN4924 likely blocks the ubiquitination of many proteins that should be destroyed (Fig. S1D), leading to the accumulation of unwanted proteins that dysregulate proteostasis and thereby interfere with CARD8 degradation. Supporting this hypothesis, we found that MLN4924 reduced the formation of CARD8 p44 , showing that it likely indirectly interferes with 20S proteasome activity in cells (Fig. S1E). Overall, our discovery that CARD8 FL K10R is functional, coupled with the lack of VbP-induced ubiquitination, strongly indicates that the 20S proteasome mediates VbP-induced pyroptosis independent of the ubiquitin-proteasome system.

Discussion
The VbP-induced proteasome pathway that degrades CARD8 has not yet been established. We previously speculated that VbP activates some unknown E3 ligase that specifically ubiquitinates the CARD8 NT fragment, thereby sending it to the 26S proteasome for destruction (8). However, no direct evidence showing that the ubiquitin-proteasome system is involved in this degradation process has emerged. Here, we show that the core 20S proteasome, which destroys unfolded or misfolded proteins independent of ubiquitin (14,15), likely controls CARD8 inflammasome activation. However, we should note that some other degradation pathways, in theory, might also be involved in CARD8 regulation. For example, proteasome complexes in addition to the isolated 20S core proteasome can degrade proteins independent of ubiquitin (18,19), and we recognize that these particles might be involved in CARD8 activation. Moreover, even though we demonstrate that CARD8 FL K10R retains the ability to mediate pyroptotic cell death, it is possible that the NT fragment is ubiquitinated on a residue other than lysine (20). Lastly, as mentioned above, it is possible that CARD8 CT ubiquitination is sufficient for activation. Further studies are needed to unequivocally rule out these mechanisms.
Our proposed model for the regulation of CARD8 by the 20S proteasome is shown in Figure 5. In unstressed cells, during normal homeostasis, the CARD8 ZU5, UPA, and CARD domains fold properly, but the N-terminal disordered region remains unstructured. The 20S proteasomes in these cells eventually degrade this disordered region. However, the well-folded ZU5 domain is too large to enter the 20S proteasome's catalytic chamber, and therefore the 20S proteasome generates the CARD8 p44 fragment. CARD8 p44 cannot form an inflammasome but can sequester CT fragments in the DPP8/9 ternary complex, further buffering unstressed cells against inappropriate CARD8 inflammasome activation. In stressed cells (e.g., VbP-or VbP + MeBs-treated cells), however, the ZU5 domain is concomitantly destroyed with the disordered region, leading to the release of the inflammatory CARD8 CT fragment. The molecular mechanisms that accelerate the degradation of the ZU5 domain are unknown, but we hypothesize that it either involves the unfolding of the ZU5 domain and/or opening of the 20S proteasome gate (21). On that note, disordered regions dramatically impair the folding of proximal domains (22), and we speculate that CARD8's disordered region, which is essential for inflammasome activation (8), might play a key role in regulating the folding of the ZU5 domain.
NLRP1 is an inflammasome-forming PRR that is closely related to CARD8 (23). NLRP1 and CARD8 share a similar ZU5-UPA-CARD region, but NLRP1 has N-terminal pyrin (PYD), nucleotide-binding (NACHT), and leucine-rich repeat (LRR) domains instead of a simple disordered sequence. We have proposed that NLRP1 and CARD8 likely both sense the same perturbation in cell homeostasis (8), but that NLRP1, which triggers a more inflammatory response than CARD8 (24), uses its NT domains to further restrain its activation (25). Based on our findings here, we speculate that the NLRP1 NT domains in some way control the rate of NT fragment destruction by the 20S proteasome. The relationship between the 20S proteasome and NLRP1 warrants further investigation.
Overall, the primordial purpose of the CARD8 inflammasome has not been definitively established. The core 20S proteasome rapidly destroys misfolded and disordered proteins but spares well-folded proteins and thereby plays a critical role in alleviating proteotoxic stress. Our findings here that the 20S proteasome controls CARD8 suggests that this inflammasome sensor monitors its own ability to fold and avoid destruction. Future studies are needed to determine why this relationship between protein folding and destruction is so closely guarded by the innate immune system.

Cell culture
HEK293T and THP-1 cells were purchased from ATCC. MV4;11 and OCI-AML2 cells were purchased from DSMZ. HEK 293T cells were grown in Dulbecco's Modified Eagle's Medium with L-glutamine and 10% fetal bovine serum. THP-1, MV4;11, and OCI-AML2 cells were grown in Roswell Park Memorial Institute medium 1640 with L-glutamine and 10% fetal bovine serum. All cells were grown at 37 C in a 5% CO 2 atmosphere incubator. Cell lines were regularly tested for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza).

Cloning
Plasmids for full-length and truncated CARD8 were cloned as described previously (8). For constitutive expressions, indicated CARD8 variants were shuttled into pLEX307 vectors that have been modified to contain different N-terminal (e.g., GFP or V5-GFP) or C-terminal (e.g., FLAG) tags using Gateway technology. For generation of CARD8 truncations (e.g., CARD8 ZUC , CARD8 Δ1-149 ), PCR was conducted with primers (with a beginning methionine) that anneal to regions within CARD8. Point mutations (e.g., CARD8 S297A, E274R) were generated using the QuikChange II site-direct mutagenesis kit (Agilent, 200523) following the manufacturer's protocol. For the generation of dTAG-CARD8 FL -V5, the Gateway-compatible   Figure 5. Model of the regulation of CARD8 by the 20S proteasome. In unstressed cells, the 20S proteasome removes the disordered region to generate CARD8 p44 , which (like CARD8 FL ) acts as an inhibitor in the DPP8/9 ternary complex (dashed blue lines). In VbP-stressed cells, the 20S degrades the entire CARD8 NT fragment, possibly due to ZU5 domain misfolding, releasing CARD8 CT from autoinhibition. Low levels of the CT fragment are captured in the ternary complex (dashed red line). Increased degradation of the full NT fragment enables the CT fragment to bypass the ternary complex (solid red line). MLN4924 blocks protein neddylation and thereby inhibits E3 ligases (solid gray line), which we speculate globally affects proteostasis and thereby interferes with 20S proteasome activity (dashed gray lines). VbP, Val-boroPro; CT, C-terminal; DPP8/9, dipeptidyl peptidase 8 or 9; NT, N-terminal; ZUC, ZU5-UPA-CARD. pLEX305 N-dTAG was used as the vector backbone to shuttle in CARD8 FL that has been attached with a GGGGS-linker sequence followed by a V5-tag and two stop codons. The DNA of the MTMR1 M1-Q94 -CARD8 ZUC construct was generated by Genscript and subsequently shuttled into the pLEX307 vector. For the generation of CARD8 M1-F161 -GFP, an assembly PCR was performed to fuse CARD8's disorder to GFP. For the generation of tetracycline (tet)-on-inducible constructs, the Gateway-compatible pINDUCER20 plasmid was used. The DNA of CARD8 lysine mutants (e.g., CARD8 K10R and K26R) were generated by Genscript and shuttled into the indicated vectors (e.g., pINDUCER20 or pLEX307). The CARD8 K10R construct have all 10 lysines N terminal to the CARD8 autoproteolysis site mutated to arginines (residues 3, 4, 9, 32, 41, 55, 147, 157, 175, 272) and for the CARD8 K26R construct, the rest of the lysines (residues 331, 345, 383, 387, 390, 404, 411, 421, 433, 455, 469, 486, 493, 498, 508, 509). Importantly, every experiment that involve CARD8 lysine mutants and their controls contain a C-terminal GGGGS linker sequence, followed by an HA-tag sequence and two stop codons prior to the Gateway attB2 recombination site. Therefore, the C-terminal sequences following CARD8's open reading frame do not code for any lysines.

Transient transfections
HEK 293T cells were plated in 6-well culture plates at 5.0 × 10 5 cells/well in Dulbecco's Modified Eagle's Medium. The next day, the indicated plasmids were added to a total of 2.0 ug DNA (with pLEX307 RFP as the filler plasmid) in 125 uL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer's protocol. Unless indicated otherwise, 2 ug of each plasmid construct was used. For experiments involving constitutive plasmid expressions, unless stated otherwise, the cells were incubated for an additional 48 h before their harvest. For experiments involving tet-inducible plasmid constructs, the cells were treated with DOX at 1 ug/ ml (and/or with other compounds) 20 h after transfection. The cells were incubated for an additional 24 h before their harvest.

CRISPR/Cas9 gene editing
To generate CARD8 knockouts in THP-1 cells, 1.5 × 10 6 cells stably expressing Cas9 (3) were infected with lentivirus containing sgRNA plasmids (packaged in HEK 293T cells using Fugene HD and 2 ug of the vector, 2 ug psPAX2, and 1 ug pMD2.G). After 48 h, cells were selected with hygromycin (100 ug/ml) until control cells died. Single cell clones were isolated by serial dilution and confirmed by Western blotting.

Generation of stable cell lines
Indicated expression plasmids (e.g., pLEX307 CARD8 FL WT HA) were packaged into lentivirus in HEK 293T cells as described above. It should be noted that all CARD8 plasmids introduced into CARD8 -/-THP-1 have silent mutations at residues F211 and S213 (generated by the Quikchange kit). 1.5 × 10 6 cells of CARD8 -/-THP-1 were then infected with the virus and after 48 h, selected with puromycin (0.5 ug/ml) until control cells died.
LDH cytotoxicity, immunoblotting, and FLAG/HA immunoprecipitation HEK 293T cells were transiently transfected and compounds treated as indicated. THP-1 cells were plated in 12-well culture plates at 0.5 × 10 6 cells/well and treated with compounds as indicated. Supernatants were analyzed for LDH levels using the Pierce LDH Cytotoxicity Assay Kit (Life Technologies). LDH levels were quantified relative to a lysis control where cells were lysed in 20 μl of a 9% Triton X-100 solution. For immunoprecipitation experiments, cell pellets were sonicated and centrifuged at 3220g for 5 min. The clarified lysates were retained and were subsequently incubated with 40 μl of anti-FLAG-M2 agarose resin (Sigma) for 1.5 h at room temperature or anti-HA agarose resin (Thermo Scientific) for overnight at 4 C. After washing with three rounds of 100 uL of PBS, bound proteins were eluted by incubating resin with 100 μl of PBS containing 150 ng/uL 3X-FLAG peptide or 1 mg/ml of HA peptide for 1 h at room temperature. An equal volume of 2× sample loading dye was added to the eluate and incubated at 95 C for 10 min. For immunoblotting, cells were washed with two rounds of PBS (pH = 7.4), resuspended in PBS that were added with 1× HALT protease inhibitor, lysed by sonication, and briefly clarified by centrifuging at 1000g for 10 min. Protein concentrations were determined and normalized using the DCA Protein Assay kit (Bio-Rad). The samples were separated by SDS-PAGE, immunoblotted, and visualized using the Odyssey Imaging System (Li-Cor).
20S proteasome assays 0.5 million HEK 293T cells were transiently transfected with 2 ug of the indicated plasmid constructs. After 48 h, the lysates were incubated with anti-FLAG or anti-HA beads before eluting with the corresponding peptides to enrich for the expressed proteins. The eluent is composed of 20 mM Tris-HCl, 20 mM NaCl, 10 mM MgCl2, and 1 mM DTT, pH 7.5. In Figure 3, A, B, D-F, the eluates were diluted with the same eluent three times before incubating with or without 200 nM of purified 20S proteasomes. In Figure 3C, the eluates were subjected to a size-exclusion filtration step (to remove excess FLAG peptide), before quantifying the protein concentration with DC protein assay (Bio-Rad). CARD8 (800 nM) was then incubated with 20S proteasomes (100 nM). All 20S reactions were incubated at 37 C for 4 h, shaking at 500 RPM before quenching with 2× loading dye prior to immunoblotting analysis.

Edman degradation
HEK 293T cells were transiently transfected with a plasmid encoding CARD8 FL WT FLAG. After 48 h, the lysates were purified with anti-FLAG beads. Then, the eluate was added with 2× loading dye and boiled, and the protein was separated by SDS-PAGE. The gel was then transferred onto a PVDF membrane and stained with Ponceau S. The bands of interest were excised and sent to the UC Davis Molecular Structure Facility for Edman sequencing analysis.

Statistical analysis
Student's two-sided t tests were performed in Figures 1,  G-I, 4, B and C, and S1, D and E. P values less than 0.05 were significant. Graphs and error bars represent means ± SD of a single experiment representative of three or more independent experiments unless stated otherwise. The investigators were not blinded in all experiments. All statistical analysis was performed using GraphPad Prism 9.

Data availability
All data in this study are available within the paper, Supporting Information, and/or from the corresponding author on reasonable request.
Supporting information-This article contains supporting information.